Kidney-derived cells and methods of use in tissue repair and regeneration
AssigneeDePuy Synthes Products, Inc.
InventorsDavid C. Colter, Agnieszka Seyda, Charito S. Buensuceso, Anna Gosiewska
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Abstract
Isolated or purified mammalian kidney-derived cell populations from mammalian kidney tissue are provided. Methods are provided for the isolation and purification of the mammalian kidney-derived cell population. Methods for treating kidney disease are provided by administration of the isolated or purified mammalian kidney-derived cell population to a mammalian subject.
Claims20
An implant comprising a thawed human kidney cell population derived from single cell clones, wherein said cell population is capable of self-renewal and expansion in culture, is positive for expression of HLA-I and CD44 and at least one of Oct-4, Rex-1, Pax-2, Cadherin-11, FoxD1, WT1, Eya1, HNF3B, CXC-R4, Sox-17, EpoR, BMP2, BMP7, or GDF5, and is negative for the expression of CD133, E-cadherin, and Wnt-4 and at least one of Sox2, FGF4, hTert, SIX2 or GATA-4;
- and a matrix comprising a biocompatible polymer, wherein the cell population is seeded on the matrix, and wherein said cell population was cryopreserved in a medium containing dimethylsulfoxide (DMSO) prior to thawing.
An implant comprising a thawed human kidney cell population derived from single cell clones, said cell population capable of self-renewal and expansion in culture, wherein the cell population is positive for expression of at least one of Oct-4, Rex-1, Pax-2, Cadherin-11, FoxD1, WT1, Eya1, HNF3B, CXC-R4, Sox-17, EpoR, BMP2, BMP7, or GDF5 and negative for the expression of at least one of Sox2, FGF4, hTert, Wnt-4, SIX2 or GATA-4 wherein the cell population secretes at least one of trophic factors FGF2, HGF, TGFα, TIMP-1, TIMP-2, MMP-2 or VEGF and does not secrete at least one of trophic factors PDGF-bb or IL12p70;
- and a matrix comprising a biocompatible polymer, wherein the cell population is seeded on the matrix, and wherein said cell population was cryopreserved in a medium containing dimethylsulfoxide (DMSO) prior to thawing.
An implant comprising a thawed human kidney cell population derived from single cell clones, said cell population capable of self-renewal and expansion in culture, wherein the cell population is positive for expression of at least one of Oct-4, Rex-1, Pax-2, Cadherin-11, FoxD1, WT1, Eya1, HNF3B, CXC-R4, Sox-17, EpoR, BMP2, BMP7, or GDF5 and negative for the expression of at least one of Sox2, FGF4, hTert, Wnt-4, SIX2 or GATA-4, wherein the cell population comprises kidney progenitor cells, and wherein the kidney progenitor cells are capable of differentiating to adipocytes or osteoblasts;
- and a matrix comprising a biocompatible polymer, wherein the cell population is seeded on the matrix, and wherein said cell population was cryopreserved in a medium containing dimethylsulfoxide (DMSO) prior to thawing.
An implant comprising a thawed human kidney cell population derived from single cell clones, said cell population capable of self-renewal and expansion in culture, wherein the cell population is positive for expression of Oct-4, Rex-1, Pax-2, Cadherin-11, FoxD1, WT1, Eya1, HNF3B, CXC-R4, Sox-17, and EpoR and negative for the expression of at least one of Sox2, FGF4, hTert, Wnt-4, SIX2 and GATA-4;
- and a matrix comprising a biocompatible polymer, wherein the cell population is seeded on the matrix, and wherein said cell population was cryopreserved in a medium containing dimethylsulfoxide (DMSO) prior to thawing.
An implant comprising a thawed human kidney cell population derived from single cell clones, said cell population capable of self-renewal and expansion in culture, wherein the cell population is positive for expression of Oct-4, Rex-1, Pax-2, Cadherin-11, FoxD1, WT1, Eya1, HNF3B, CXC-R4, Sox-17, and EpoR, negative for the expression of at least one of Sox2, FGF4, hTert, Wnt-4, SIX2 and GATA-4;
- positive for cell-surface markers HLA I, CD24, CD29, CD44, CD49c, CD73, CD90, CD166, and SSEA-4;
- and negative for cell-surface markers HLA II, CD31, CD34, CD45, CD56, CD80, CD86, CD104, CD105, CD117, CD133, CD138, CD141, and E-cadherin;
- and a matrix comprising a biocompatible polymer, wherein the cell population is seeded on the matrix, and wherein said cell population was cryopreserved in a medium containing dimethylsulfoxide (DMSO) prior to thawing.
An implant comprising a thawed human kidney cell population derived from single cell clones, said cell population capable of self-renewal and expansion in culture, wherein the cell population is positive for expression of Oct-4, Rex-1, Pax-2, Cadherin-11, FoxD1, WT1, EpoR, BMP2, BMP7, and GDF5, and negative for the expression of Sox2, FGF4, and hTert;
- and a matrix comprising a biocompatible polymer, wherein the cell population is seeded on the matrix, and wherein said cell population was cryopreserved in a medium containing dimethylsulfoxide (DMSO) prior to thawing.
Dependencies are inferred from the claim text.