Cell line stably expressing Nav1.5 and method of screening therapeutic agent using the cell line
AssigneeThe Catholic University of Korea Industry-Academic Cooperation Foundation
InventorsJin-Sung Choi, Jeong Hee Choi
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Abstract
Provided are a cell line stably expressing voltage-gated sodium channel (Nav1.5), wherein a SCN5A gene (NCBI accession no. NM_198056) encoding Nav1.5 and a puromycin selection marker are inserted into a chromosome of a host cell, and a method of measuring cardiotoxicity of a test substance comprising: (1) contacting a test substance to the cell line of claim 1; and (2) measuring magnitude of voltage-gated sodium channel (Nav1.5) in the cell line in contact with the test substance.
Claims5
A cell line stably expressing voltage-gated sodium channel (Nav1.5), the cell line having accession number KCTC 13095BP, wherein a SCN5A gene consisting of NCBI accession no. NM_198056 encoding Nav1.5 and a puromycin selection marker are inserted into a chromosome of the cell.
Dependencies are inferred from the claim text.