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US 10,481,077 B1Granted
Cell capture system and method of use
AssigneeCelsee Diagnostics, Inc.
InventorKalyan Handique
Life of this patent
Estimating the term…
Abstract
A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.
Claims20
A method comprising:
- providing a fluidic network comprising an inlet channel at an upstream end, one or more outlet channels at a downstream end, and a set of structures in fluid communication with the inlet channel and the one or more outlet channels, wherein flow from the inlet channel is configured to reach at least one of the one or more outlet channels only by way of the set of structures;
- receiving a fluid sample comprising a set of target cells into the fluidic network;
- capturing and partitioning the set of target cells in single-cell format, by way of the set of structures, wherein capturing and partitioning comprises isolating a target cell of the set of target cells in single-cell format with an isolation material within at least a portion of the fluidic network;
- and
- transmitting material associated with the set of target cells from the fluidic network.
2 more elements
A method comprising:
- providing a fluidic network comprising an inlet channel, an outlet channel, and a set of structures in fluid communication with the inlet channel and one or more outlet channels, wherein flow from the inlet channel is configured to reach at least one of the one or more outlet channels only by way of the set of structures;
- receiving a fluid sample comprising a set of target cells into the fluidic network;
- capturing and partitioning the set of target cells in single-cell format, by way of the set of structures, wherein capturing and partitioning comprises isolating a target cell of the set of target cells in single-cell format with an isolation material within at least a portion of the fluidic network;
- generating cellular lysate upon lysing the target cell with a lysis reagent;
- and
- performing a reaction with the cellular lysate.
3 more elements
Dependencies are inferred from the claim text.