Basophil analysis system and method
AssigneeAbbott Laboratories
InventorsJiong Wu, Michael R. Buhl, Giacomo Vacca
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Abstract
Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements.
Claims21
A hematology analyzer for conducting a basophil analysis on a blood sample that contains a plurality of basophils and lymphocytes, the analyzer comprising:
- an excitation source positioned to excite particles within the blood sample;
- a detector unit comprising a fluorescence detector positioned to measure fluorescence emitted from the excited blood sample;
- and
- a processor;
- a non-transitory computer-readable memory medium comprising instructions that cause the processor to:
- excite the blood sample with an excitation source in a flow cell;
- collect a plurality of fluorescence emission signals from the excited sample;
- prior to performing a basophil analysis, exclude nuclei-free events and retain nuclei-containing events using only a fluorescence trigger that is limited to fluorescence emission signals and is set to a fluorescence magnitude that is greater than fluorescence emission signals from RBCs, including RBC fragments, and is less than fluorescence emission signals from white blood cells (WBCs);
- and
- perform a basophil cluster analysis on the nuclei-containing events.
Dependencies are inferred from the claim text.