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US 10,481,068 B2Granted
Hydrogel particles with tunable optical properties
AssigneeSlingshot Biosciences, Inc.
InventorsJeffrey Kim, Oliver Liu, Jeremy Agresti, Anh Tuan Nguyen
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Abstract
The present disclosure relates to compositions comprising a hydrogel particle with optical properties substantially similar to the optical properties of a target cell, and methods for their use.
Claims27
A method, comprising:
- calibrating a cytometric device for analysis of a human target cell, the calibrating including:
- inserting hydrogel particles having optical scatter properties at least 70% similar to optical scatter properties of the human target cell into the cytometric device, a physical property of the hydrogel particles being closer in value to a corresponding physical property of the human target cell than a corresponding physical property of polystyrene beads having substantially the same average diameter as the hydrogel particles, the optical scatter properties of the hydrogel particles provided by:
- a co-monomer, a chemical side-group, an encapsulated material, a colloidal silica, or a ratio of acrylamide to bi-acrylamide,
- measuring the physical property of the hydrogel particles using the cytometric device, and
- calibrating the cytometric device based on the measurements of the hydrogel particles;
- and
- analyzing the human target cell with the calibrated cytometric device.
4 more elements
A method of calibrating a measurement device for analysis of a human target cell, the method comprising:
- a) inserting into the measurement device hydrogel particles having optical scatter and/or biochemical properties at least 70% similar to optical scatter and/or biochemical properties of a human target cell type, a material modulus property of the hydrogel particles being closer in value to a corresponding material modulus property of the human target cell than a corresponding material modulus property of polystyrene beads having substantially the same average diameter as the hydrogel particles, the optical scatter properties of the hydrogel particles provided by:
- a co-monomer, a chemical side-group, an encapsulated material, a colloidal silica, or a ratio of acrylamide to bi-acrylamide;
- b) measuring the properties of the hydrogel particles using the measurement device;
- and c) calibrating the measurement device based on the measurements of the hydrogel particles.
1 more element
Dependencies are inferred from the claim text.