System and method for refilling cryogen in microscope cryogen holders

A cryogen refilling system for a microscope cryogen holder, comprising:

• a reservoir assembly for holding cryogen;
• a delivery channel in fluid communication with the reservoir assembly for delivering cryogen from the reservoir assembly to a microscope cryogen holder, wherein the delivery channel comprises at least one control valve;
• a sensor-lid assembly comprising a sensor assembly configured to detect sensor data corresponding to a level of cryogen in the microscope cryogen holder, wherein the sensor assembly is configured to have a reentrant tube design;
• and
• a controller, wherein the controller comprises:
• a processor in communication with the sensor assembly, and
• a computer-readable medium containing programming instructions that are configured to, when executed by the processor, cause the processor to:
• periodically, cause a valve control mechanism to open the at least one control valve of the delivery channel to deliver cryogen to the microscope cryogen holder,
• receive sensor data from the sensor assembly,
• analyze the sensor data to determine whether cryogen level in the microscope cryogen holder has reached a threshold level, and
• in response to determining that the cryogen level in the microscope cryogen holder has reached the threshold level, cause the valve control mechanism to close the at least one control valve of the delivery channel.

A cryogen refilling system for a microscope cryogen holder, comprising:

• a reservoir assembly for holding cryogen;
• a delivery channel in fluid communication with the reservoir assembly for delivering cryogen from the reservoir assembly to a microscope cryogen holder, wherein the delivery channel comprises at least one control valve;
• a sensor-lid assembly comprising a sensor assembly configured to detect sensor data corresponding to a level of cryogen in the microscope cryogen holder, wherein the sensor assembly is configured to have a reentrant tube design;
• and
• a controller, wherein the controller comprises:
• a processor in communication with the sensor assembly, and
• a computer-readable medium containing programming instructions that are configured to, when executed by the processor, cause the processor to:
• receive sensor data from the sensor assembly,
• analyze the sensor data to determine whether the microscope cryogen holder requires refilling, and
• in response to determining that the microscope cryogen holder requires refilling, cause a valve control mechanism to open the at least one control valve of the delivery channel.

A cryogen refilling system for a microscope cryogen holder, comprising:

• a reservoir assembly for holding cryogen;
• a delivery channel in fluid communication with the reservoir assembly for delivering cryogen from the reservoir assembly to a microscope cryogen holder, wherein the delivery channel comprises at least one control valve;
• a sensor-lid assembly comprising a sensor assembly configured to detect sensor data corresponding to a level of cryogen in the microscope cryogen holder;
• a support frame, wherein:
• the support frame comprises an arm segment and a vertical segment, and
• the reservoir assembly is mounted on the arm segment via a sliding unit configured to move the reservoir assembly along an axis parallel to a length of the arm segment;
• and
• a controller, wherein the controller comprises:
• a processor in communication with the sensor assembly, and
• a computer-readable medium containing programming instructions that are configured to, when executed by the processor, cause the processor to:
• receive sensor data from the sensor assembly,
• analyze the sensor data to determine whether the microscope cryogen holder requires refilling, and
• in response to determining that the microscope cryogen holder requires refilling, cause a valve control mechanism to open the at least one control valve of the delivery channel.

A cryogen refilling system for a microscope cryogen holder, comprising:

• a reservoir assembly for holding cryogen;
• a delivery channel in fluid communication with the reservoir assembly for delivering cryogen from the reservoir assembly to a microscope cryogen holder, wherein the delivery channel comprises at least one control valve;
• a sensor-lid assembly comprising a sensor assembly configured to detect sensor data corresponding to a level of cryogen in the microscope cryogen holder;
• and
• a controller, wherein the controller comprises:
• a processor in communication with the sensor assembly, and
• a computer-readable medium containing programming instructions that are configured to, when executed by the processor, cause the processor to:
• receive sensor data from the sensor assembly,
• analyze the sensor data to determine whether the microscope cryogen holder requires refilling, and
• in response to determining that the microscope cryogen holder requires refilling:
• cause movement of at least one component of the cryogen refilling system such that the reservoir assembly is positioned to supply liquid cryogen to the microscope cryogen holder, and
• upon positioning of the reservoir assembly, cause a valve control mechanism to open the at least one control valve of the delivery channel.