Sensors and methods of manufacture thereof
AssigneePurdue Research Foundation
InventorsRajtarun Madangopal, Jenna L. Rickus
Life of this patent
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Abstract
The invention generally relates to sensors, methods of manufacture thereof, methods of use thereof for sensing analytes, such as small molecules and biomolecules, and methods of immobilization. In certain embodiments, the invention provides a multi-analyte sensor. The multi-analyte sensor includes a plurality of sensing electrodes. Each sensing electrode is functionalized with a different molecule (e.g., biomolecule), at least two of the sensing electrodes are spaced apart prior to and after functionalization by 100 μm or less, and there is no cross-talk between the plurality of sensing electrodes.
Claims20
A method for detecting one or more target biological molecules in a sample, the method comprising:
- providing a multi-analyte sensor that comprises a plurality of sensing electrodes, each sensing electrode comprising at least one electrically conductive material and being functionalized with a different enzyme composition from other sensing electrodes of the plurality, wherein at least two of the plurality of sensing electrodes are spaced apart at a microscale level prior to and after functionalization and there is negligible cross-talk between the plurality of sensing electrodes, wherein each sensing electrode comprises:
- a first layer comprising a first electrically conductive material, a second layer comprising a second electrically conductive material, and a third layer comprising a third electrically conductive material;
- wherein conductivity of the third electrically conductive material is pH dependent, and wherein the first layer is platinum black, the second electrically conductive biocompatible material is poly(3,4-ethylenedioxythiophene), and the third electrically conductive material is poly(o-aminophenol);
- contacting a sample to the multi-analyte sensor;
- and
- detecting one or more target biological molecules from the sample, wherein each of the one or more target biological molecules is detected on a different sensing electrode of the plurality of sensing electrodes.
A method for detecting one or more target biological molecules in a sample, the method comprising:
- providing a microchip that comprises a plurality of sensing electrodes, each sensing electrode comprising at least one electrically conductive material and being functionalized with a different enzyme composition from other sensing electrodes of the plurality, wherein at least two of the plurality of sensing electrodes are spaced apart at a microscale level prior to and after functionalization and there is negligible cross-talk between the plurality of sensing electrodes, wherein each sensing electrode comprises:
- a first layer comprising a first electrically conductive material, a second layer comprising a second electrically conductive material, and a third layer comprising a third electrically conductive material;
- wherein conductivity of the third electrically conductive material is pH dependent, and wherein the first layer is platinum black, the second electrically conductive biocompatible material is poly(3,4-ethylenedioxythiophene), and the third electrically conductive material is poly(o-aminophenol);
- contacting a sample to the microchip;
- and
- detecting one or more target biological molecules from the sample, wherein each of the one or more target biological molecules is detected on a different sensing electrode of the plurality of sensing electrodes.
Dependencies are inferred from the claim text.