Methods of detecting influenza
AssigneeCepheid
InventorsAnupama Mokkapati, Bradley Brown, Robert Jones
Life of this patent
Estimating the term…
Abstract
Compositions and methods for detecting influenza are provided.
Claims11
A kit for detecting the presence of influenza in a sample from a human subject comprising
- (a) first primer pair for detecting an influenza A PA gene, wherein the first primer pair comprises a first and second primer, wherein
- the first primer comprises a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 20;
- and the second primer comprises a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 21;
- (b) a second primer pair for detecting an influenza A PB2 gene, wherein the second primer pair comprises a third and fourth primer, wherein
- the third primer comprises a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 17, and the fourth primer comprises a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 18;
- (c) a third primer pair for detecting an influenza A MP gene, wherein the third primer pair comprises a fifth and sixth primer, wherein
- the fifth primer comprises a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 23, and the sixth primer comprises a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 24;
- (d) a fourth primer pair for detecting an avian influenza MP gene, wherein the fourth primer pair comprises a seventh and eighth primer, wherein
- the seventh primer comprises a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 26, and the eighth primer comprises a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 27;
- and
- (e) at least one probe selected from an influenza A PA probe comprising a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 22, an influenza A PB2 probe comprising a sequence that is at least 90% identical or complementary to at least 15 contiguous nucleotides of SEQ ID NO:
- 19, an influenza A MP probe comprising a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 25, and an avian influenza MP probe comprising a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 28.
A composition comprising a primer pair comprising a first primer comprising a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 20;
- and a second primer comprising a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 21;
- and a probe comprising a sequence that is at least 90% identical or complementary to at least 15 contiguous nucleotides of SEQ ID NO:
- 22.
A composition comprising a primer pair comprising a first primer comprising a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 17;
- and a second primer comprising a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 18;
- and a probe comprising a sequence that is at least 90% identical or complementary to at least 15 contiguous nucleotides of SEQ ID NO:
- 19.
A composition comprising a primer pair comprising a first primer comprising a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 23;
- and a second primer comprising a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 24;
- and a probe comprising a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 25.
A composition comprising a primer pair comprising a first primer comprising a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 26;
- and a second primer comprising a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 27;
- and a probe comprising a sequence that is at least 90% identical to at least 15 contiguous nucleotides of SEQ ID NO:
- 28.
Dependencies are inferred from the claim text.