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US 10,480,016 B2Granted
Genetically engineered microorganisms for biological oxidation of hydrocarbons
AssigneeCalysta, Inc.
InventorsJoshua A. Silverman, Drew D. Regitsky
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Abstract
The present disclosure relates to genetically engineered microorganisms for biological oxidation of hydrocarbons, including production of alcohols from alkanes or epoxides from alkenes, and related methods and systems.
Claims16
A non-naturally occurring bacterium, comprising:
- a host methanotrophic bacterium having an exogenous nucleic acid encoding an enzyme with methane monooxygenase (MMO) activity,
- wherein the MMO comprises an amino acid sequence having at least 90% identity to the amino acid sequence of any one of SEQ ID NOS:
- 99-102, 174, 197, and 649-653;
- wherein at least one alcohol dehydrogenase enzyme is inactivated in the non-naturally occurring bacterium as compared to the host methanotrophic bacterium;
- and
- wherein the non-naturally occurring bacterium is capable of converting methane into methanol.
3 more elements
Dependencies are inferred from the claim text.