Back to results
US 10,480,009 B2Granted
Biological state machines
AssigneeMassachusetts Institute of Technology
InventorsTimothy Kuan-Ta Lu, Nathaniel B. Roquet
Life of this patent
Estimating the term…
Abstract
Provided herein are recombinase-based frameworks for building state machines in vitro and in vivo by using chemically controlled DNA excision and inversion operations to encode state in DNA sequence.
Claims14
A system, comprising:
- (a) n serine recombinases, wherein n is greater than 2;
- and
- (b) an engineered nucleic acid comprising n(n−1) pairs of cognate recombination recognition sites (RRS),
- wherein the n(n−1) pairs of RRSs of (b) are arranged in an overlapping configuration such that the two RRSs of each pair of the n(n−1) pairs of RRSs are separated from each other by at least one RRS of another pair of the n(n−1) pairs of RRSs, and wherein recombination between the two RRSs of each pair of the n(n−1) pairs of RRSs either inverts or excises at least one RRS of another pair of the n(n−1) pairs of RRSs.
1 more element
A system, comprising:
- (a) three serine recombinases;
- and
- (b) an engineered nucleic acid comprising six pairs of cognate recombinase recognition sites (RRSs),
- wherein the six pairs of RRSs of (b) are arranged in an overlapping configuration such that the two RRSs of each pair of the six pairs of RRSs are separated from each other by at least one RRS of another pair of the six pairs of RRSs, and wherein recombination between the two RRSs of each pair of the six pairs of RRSs either inverts or excises at least one RRS of another pair of the six pairs of RRSs.
1 more element
A system, comprising:
- (a) four serine recombinases;
- and
- (b) an engineered nucleic acid comprising twelve pairs of cognate recombinase recognition sites (RRSs),
- wherein the twelve pairs of RRSs of (b) are arranged in an overlapping configuration such that the two RRSs of each pair of the twelve pairs of RRSs are separated from each other by at least one RRS of another pair of the twelve pairs of RRSs, and wherein recombination between the two RRSs of each pair of the twelve pairs of RRSs either inverts or excises at least one RRS of another pair of the twelve pairs of RRSs.
1 more element
Dependencies are inferred from the claim text.