Insecticidal polypeptides having broad spectrum activity and uses thereof

An isolated polypeptide operably linked to a heterologous signal peptide, wherein the isolated polypeptide is selected from the group consisting of:

• (a) a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
• 2 and SEQ ID NO:
• 4;
• (b) a polypeptide that is encoded by a nucleotide sequence selected from the group consisting of SEQ ID NO:
• 1 and SEQ ID NO:
• 3;
• (c) a polypeptide sequence that differs by 1 to 3 amino acid resides compared to the polypeptide sequence of SEQ ID NO:
• 2, wherein the polypeptide has insecticidal activity;
• and
• (d) a polypeptide sequence that differs by 1 to 3 amino acid resides compared to the polypeptide sequence of SEQ ID NO:
• 2, wherein the polypeptide of (a), (b), (c) or (d) has insecticidal activity against soybean looper.

A composition comprising an agriculturally acceptable carrier and a polypeptide operably linked to a heterologous signal peptide, wherein the polypeptide is selected from the group consisting of:

• a) a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
• 2 and SEQ ID NO:
• 4;
• (b) a polypeptide that is encoded by a nucleotide sequence selected from the group consisting of SEQ ID NO:
• 1 and SEQ ID NO:
• 3;
• (c) a polypeptide sequence that differs by 1 to 3 amino acid resides compared to the polypeptide sequence of SEQ ID NO:
• 4, wherein the polypeptide has insecticidal activity against soybean looper;
• and
• (d) a polypeptide sequence that differs by 1 to 3 amino acid resides compared to the polypeptide sequence of SEQ ID NO:
• 2, wherein the polypeptide has insecticidal activity against soybean looper.

A plant having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having pesticidal activity, wherein said nucleotide sequence is selected from the group consisting of:

• (a) a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
• 1 and SEQ ID NO:
• 3;
• (b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence selected from the group consisting of SEQ ID NO:
• 2 and SEQ ID NO:
• 4;
• (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence that differs by 1 to 3 amino acid resides compared to the amino acid sequence of-SEQ ID NO:
• 4, wherein the polypeptide has insecticidal activity;
• and
• (d) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence that differs by 1 to 3 amino acid resides compared to the amino acid sequence of SEQ ID NO:
• 2, wherein the polypeptide has insecticidal activity;
• wherein said nucleotide sequence is operably linked to a promoter that drives expression of a coding sequence in a plant cell.

A method for protecting a plant from a pest, comprising introducing into said plant or cell thereof at least one expression vector comprising a nucleotide sequence that encodes a pesticidal polypeptide, wherein said nucleotide sequence is selected from the group consisting of:

• (a) a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:
• 1 and SEQ ID NO:
• 3;
• (b) a nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:
• 2 and SEQ ID NO:
• 4;
• (c) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence that differs by 1 to 3 amino acid resides compared to the amino acid sequence of SEQ ID NO:
• 4, wherein the polypeptide has insecticidal activity;
• and
• (d) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence that differs by 1 to 3 amino acid resides compared to amino acid sequence of SEQ ID NO:
• 2, wherein the polypeptide has insecticidal activity.