Brassica plant comprising a mutant ALCATRAZ allele
AssigneeBASF Agricultural Solutions Seed US LLC
InventorBenjamin Laga
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Abstract
The present invention relates to Brassica plants comprising mutant ALC genes, ALC nucleic acid sequences and proteins, as well as methods for generating and identifying said plants and alleles, which can be used to plants with increased podshatter resistance.
Claims9
A Brassica napus plant, or a cell, part, seed or progeny thereof, comprising two non-naturally occurring full knock-out alc genes, wherein one non-naturally occurring full knock-out alc gene is a mutated version of the native ALC gene comprising at least 98% sequence identity to SEQ ID NO:
- 3 or encoding an amino acid sequence comprising at least 98% sequence identity to SEQ ID NO:
- 9;
- and wherein one non-naturally occurring full knock-out alc gene is a mutated version of the native ALC gene comprising at least 98% sequence identity to SEQ ID NO:
- 4 or encoding an amino acid sequence comprising at least 98% sequence identity to SEQ ID NO:
- 10;
- wherein said full knock-out mutant alc gene comprises a mutated DNA region consisting of one or more inserted, deleted or substituted nucleotides compared to a corresponding wild-type DNA region in the functional ALC gene and wherein said mutant alc allele does not encode a functional ALC protein,
- wherein the podshatter resistance is increased as compared to the podshatter resistance of a corresponding plant not comprising full knock-out alc genes.
A full knock-out allele of an ALC gene, wherein the ALC gene from A genome contains a mutated splice site characterized by a G to A substitution at position 668 of SEQ ID NO:
- 3, and wherein the ALC gene from C genome contains a premature stopcodon characterized by a C to T substitution at position 646 of SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.