RNA duplexes with single stranded phosphorothioate nucleotide regions for additional functionality
AssigneePhio Pharmaceuticals Corp.
InventorsTod M. Woolf, Joanne Kamens, William Salomon, Anastasia Khvorova
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Abstract
The present invention relates to RNAi constructs and their use in gene silencing. RNAi constructs associated with the invention contain a double stranded region connected to a single stranded region of phosphorothioate modified nucleotides.
Claims20
An isolated double stranded nucleic acid molecule comprising a guide strand and a passenger strand, wherein the passenger strand is 8-16 continuous nucleotides in length, wherein the isolated double stranded nucleic acid molecule comprises a double stranded region that is 8-16 nucleotides in length, wherein the guide strand is 19-25 nucleotides in length, and wherein the double stranded region is connected through a cleavable chemical linker to a single stranded region of at least six phosphorothioate modified nucleotides.
An isolated double stranded nucleic acid molecule comprising a guide strand and a passenger strand, wherein the passenger strand is 8-16 continuous nucleotides in length, wherein the isolated double stranded nucleic acid molecule comprises a double stranded region that is 8-16 nucleotides in length, wherein the guide strand is 19-25 nucleotides in length, and wherein the double stranded region is connected through a cleavable chemical linker to a single stranded region of at least three phosphorothioate modified nucleotides.
Dependencies are inferred from the claim text.