Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsLan Tang, Xianzhi Jiang, Bucong Han, Christine Kai, Ye Liu
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Abstract
Disclosed herein are polypeptides having cellobiohydrolase activity, catalytic domains, carbohydrate binding modules and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding modules. Disclosed herein are nucleic acid constructs, vectors and host cells comprise the polynucleotides as well as their uses, and methods of producing the polypeptides, catalytic domains, carbohydrate binding modules.
Claims45
A polypeptide comprising a carbohydrate binding module operably linked to a catalytic domain, wherein the carbohydrate binding module is heterologous to the polypeptide, and wherein the carbohydrate binding module is selected from the group consisting of:
- (a) a carbohydrate binding module having at least 95% sequence identity to amino acids 517 to 545 of SEQ ID NO:
- 2;
- (b) a carbohydrate binding module comprising amino acids 517 to 545 of SEQ ID NO:
- 2;
- and
- (c) a carbohydrate binding module encoded by a polynucleotide having at least 98% sequence identity to nucleotides 1549 to 1635 of SEQ ID NO:
- 1.
A nucleic acid construct or expression vector comprising a polynucleotide encoding a polypeptide having cellobiohydrolase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in a recombinant host cell, and wherein the polypeptide having cellobiohydrolase activity is selected from the group consisting of:
- (a) a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide having at least 98% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1;
- (c) a polypeptide comprising a catalytic domain having at least 95% sequence identity to amino acids 26 to 464 of SEQ ID NO:
- 2;
- and
- (d) a polypeptide comprising a catalytic domain encoded by a polynucleotide having at least 98% sequence identity to nucleotides 76 to 1392 of SEQ ID NO:
- 1.
A process for degrading a cellulosic or hemicellulosic material, comprising:
- treating the cellulosic or hemicellulosic material with an enzyme composition comprising a polypeptide having cellobiohydrolase activity, wherein the polypeptide having cellobiohydrolase activity is selected from the group consisting of:
- (a) a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide having at least 98% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1;
- (c) a polypeptide comprising a catalytic domain having at least 95% sequence identity to amino acids 26 to 464 of SEQ ID NO:
- 2;
- and
- (d) a polypeptide comprising a catalytic domain encoded by a polynucleotide having at least 98% sequence identity to nucleotides 76 to 1392 of SEQ ID NO:
- 1.
A process for producing a fermentation product, comprising:
- (a) saccharifying a cellulosic or hemicellulosic material with an enzyme composition comprising a polypeptide having cellobiohydrolase activity, wherein the polypeptide having cellobiohydrolase activity is selected from the group consisting of:
- (i) a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- (ii) a polypeptide encoded by a polynucleotide having at least 98% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1;
- (iii) a polypeptide comprising a catalytic domain having at least 95% sequence identity to amino acids 26 to 464 of SEQ ID NO:
- 2;
- and
- (iv) a polypeptide comprising a catalytic domain encoded by a polynucleotide having at least 98% sequence identity to nucleotides 76 to 1392 of SEQ ID NO:
- 1;
- (b) fermenting the saccharified cellulosic or hemicellulosic material with one or more fermenting microorganisms to produce the fermentation product;
- and
- (c) recovering the fermentation product from the fermentation.
A process of fermenting a cellulosic or hemicellulosic material, comprising:
- fermenting the cellulosic or hemicellulosic material with one or more fermenting microorganisms, wherein the cellulosic or hemicellulosic material is saccharified with an enzyme composition comprising a polypeptide having cellobiohydrolase activity, wherein the polypeptide having cellobiohydrolase activity is selected from the group consisting of:
- (a) a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide having at least 98% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1;
- (c) a polypeptide comprising a catalytic domain having at least 95% sequence identity to amino acids 26 to 464 of SEQ ID NO:
- 2;
- and
- (d) a polypeptide comprising a catalytic domain encoded by a polynucleotide having at least 98% sequence identity to nucleotides 76 to 1392 of SEQ ID NO:
- 1.
A recombinant host cell transformed with a nucleic acid construct or an expression vector comprising a polynucleotide encoding a polypeptide having cellobiohydrolase activity, wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in the recombinant host cell, wherein the polypeptide having cellobiohydrolase activity is heterologous to the recombinant host cell, and wherein the polypeptide having cellobiohydrolase activity is selected from the group consisting of:
- (a) a polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide having at least 98% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1;
- (c) a polypeptide comprising a catalytic domain having at least 95% sequence identity to amino acids 26 to 464 of SEQ ID NO:
- 2;
- and
- (d) a polypeptide comprising a catalytic domain encoded by a polynucleotide having at least 98% sequence identity to nucleotides 76 to 1392 of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.