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AssigneeDanisco US Inc.
InventorsNicholai Douglas, Maria Foukaraki, Ronaldus Wilhelmus Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Suzanne E. Lantz, Jonathan K. Lassila, Igor Nikolaev, Daniel Esteban Torres-Pazmino, Wilhelmus Van Der Kley, Johannes Franciscus Thomas Van Lieshout, Sander Van Stigt Thans
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Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.
A variant glycoside hydrolase family 61 (GH61) polypeptide, wherein said variant has cellulase enhancing activity, has at least 95% sequence identity to the amino acid sequence of SEQ ID NO:3 and the amino acid substitution I114L, and has at least one improved property relative to a parent GH61 polypeptide, wherein said improved property is selected from the group consisting of (a) expression based on protein level and RNA level or activity of the variant GH61 polypeptide, (b) thermostability and/or melting temperature (Tm), (c) performance in a Whole Hydrolysate Dilute Acid Pretreated Corn Stover (whPCS) hydrolysis assay.
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