Postnatal adherent cells and preparation method therefor

A method of preparing enhanced postnatal adherent cells, the method comprising:

• obtaining an amniotic tissue from a detached placenta;
• harvesting cell populations by adding an enzyme mixed solution to the amniotic tissue, wherein the enzyme mixed solution comprises collagenase at a concentration of 0.5 mg/ml to 5 mg/ml, trypsin at a concentration of 1 mg/ml to 5 mg/ml, DNA hydrolase (DNase) at a concentration of 0.01 mg/ml to 0.05 mg/ml, and dispase at a concentration of 0.1 U/ml to 5 U/ml;
• isolating the enhanced postnatal adherent cells by culturing the harvested cell populations in a container by adhesion culture and then treating the cell populations with an animal component-free (ACF) recombinant enzyme;
• and
• subculturing the isolated enhanced postnatal adherent cells under a hypoxia condition lower than a normoxia condition of 21% in a medium containing fibroblast growth factor-4 (FGF-4) and heparin at least 6 passages, wherein the subculturing further comprises treatment of the animal component-free recombinant enzyme before transferring the cells for subculturing at every stage of subculturing, and
• wherein 80% or more of the adherent cells express CD44, CD73, CD90, and CD105-positive surface markers and 10% or less thereof express a CD45-negative surface marker, and more vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) are secreted compared to the isolated enhanced postnatal adherent cells of passages 1 to 4.