Method, device and kit for mass cultivation of cells using polyimide porous membrane

A mass cell culturing method including:

• (1) applying cells to a porous polyimide film, and
• (2) applying the porous polyimide film to which the cells have been applied, to a cell culture medium and performing culturing the porous polyimide film to which the cells have been applied, until the number of cells reaches 1.0×106 or more per milliliter of medium, or until the number of cells per square centimeter of the porous polyimide film reaches 1.0×105,
• wherein the porous polyimide film has a multilayer structure having at least two surface layers (an A-surface and a B-surface), and a macro-void layer sandwiched between the two surface layers,
• a mean pore size of the holes in the A-surface is smaller than a mean pore size of the holes in the B-surface, and
• the macro-void layer has a partition bonded to the surface layers (the A-surface and the B-surface), and a plurality of macro-voids surrounded by the partition and the surface layers (the A-surface and the B-surface).