A biochip device for detection of biologic molecules in a fluidic sample, comprising:
- (1) a plastic substrate, having a variety of microfluidic structures including a detection zone groove, and a closed area for collecting the fluidic sample, wherein a plurality of golden fingers are located at an edge of the plastic substrate and extended with convergent spacing to an edge of the detection zone groove;
- (2) at least one integrated circuit (IC) chip embedded in the detection zone groove of the plastic substrate, the IC chip having at least one detection structure made of a sensing material for generating a detecting signal, and I/O pads for connecting an external power source and outputting the detecting signal, in which the at least one detection structure is a modified structure modified by using biological conjugates for measuring biologic molecules in the fluidic sample, the I/O pads of the IC chip are wire bonded to the plurality of golden fingers on the edge of the plastic substrate;
- (3) a sealing cover made of Polydimethylsiloxane (PDMS) for sealing the plastic substrate embedded with the IC chip, being disposed on the plastic substrate with its bottom side facing the substrate, wherein there are an inlet region for loading the fluidic sample, a separation structure, microfluidic channels, a structure for slowing the flow of the fluidic sample, and a reaction region on the sealing cover;
- and wherein there is lateral space formed between the detection zone groove and the closed area for collecting the fluidic sample, and the lateral space spans between the plastic substrate and the bottom side of the sealing cover;
- and wherein the microfluidic channels are connected from the inlet region, the separation structure for slowing the flow of the fluidic sample, the reaction region to the closed area for collecting the fluidic sample;
- and
- (4) a vacuum bag encapsulating the plastic substrate embedded with the IC chip and covered by the sealing cover, whereby the microfluidic structures in the plastic substrate are kept in vacuum state inside the vacuum bag;
- wherein the biochip device is ready for detection, and when the vacuum bag is unpacked, the fluidic sample loaded at the inlet region flows through the microfluidic channels by degas-driven flow and to the at least one detection structure at the IC chip and wherein the volume of the fluidic sample flowing to the closed area for collecting the fluidic sample is fixed and predetermined so as to define a given volume of the fluidic sample to be detected.