Method for preparing dendritic cell, dendritic cell prepared thereby, and use thereof

A method for preparing a dendritic cell, comprising:

• culturing an immature dendritic cell to a mature dendritic cell with treating by 1-10 ng/mL of rapamycin and a maturation factor;
• and
• after maturation of the immature dendritic cell to the mature dendritic cell has been completed, sensitizing the mature dendritic cell with an antigen bound to a peptide having cell permeability,
• wherein said sensitizing of the mature dendritic cell with the antigen bound to the peptide having cell permeability is performed at least 6 hours and up to 48 hours after said treating by the maturation factor, and
• wherein said sensitizing of the mature dendritic cell with the antigen bound to the peptide having cell permeability is performed for 1 to 8 hours.