Chimeric protein
AssigneeUCL Business PLC
InventorsMartin Pulé, Ryan Trowbridge, Edward Hodgkin
Life of this patent
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Abstract
The present invention provides a chimeric protein having the formula: Casp-Ht1-Ht2 wherein Casp is a caspase domain; Ht1 is a first heterodimerization domain; and Ht2 is a second heterodimerization domain and wherein, in the presence of a chemical inducer of dimerization (CID), an identical pair of the chimeric proteins interact such that Ht1 from one chimeric protein heterodimerizes with Ht2 from the other chimeric protein, causing homodimerization of the two caspase domains. The invention also provides a cell comprising such a protein and its use in adoptive cell therapy.
Claims13
A chimeric protein having the formula:
- Ht1-Ht2-Casp, wherein
- Casp is a caspase domain;
- Ht1 is a first heterodimerization domain;
- and
- Ht2 is a second heterodimerization domain;
- wherein one heterodimerization domain comprises an FK506-binding protein (FKBP) and the other heterodimerization domain comprises an FRB domain of mTOR;
- and wherein, in the presence of rapamycin or a rapamycin analog, an identical pair of the chimeric proteins interact such that Ht1 from one chimeric protein heterodimerizes with Ht2 from the other chimeric protein, causing homodimerization of the two caspase domains.
A nucleic acid comprising a nucleotide sequence which encodes a chimeric protein having the formula:
- Ht1-Ht2-Casp,
- wherein Casp is a caspase domain;
- Ht1 is a first heterodimerization domain;
- and Ht2 is a second heterodimerization domain;
- wherein one heterodimerization domain comprises an FK506-binding protein (FKBP) and the other heterodimerization domain comprises an FRB domain of mTOR.
Dependencies are inferred from the claim text.