Microfluidic chip with sealed on-board reagent

A microfluidic chip, comprising:

• a housing;
• a sample port supported by the housing for introducing fluids into the housing;
• one or more channels providing fluid communication between the sample port and a detection chamber;
• and,
• one or more product pouch assemblies positioned adjacent to the one or more channels and housing one or more reagents;
• wherein the product pouch assemblies have a pouch forming an inner chamber, at least a portion of the pouch having a rupturing portion, the rupturing portion positioned in fluid communication with the one or more reagents and the one or more channels,
• wherein the inner chamber includes two or more cavities, the cavities separated by an inner membrane,
• wherein one of the two or more cavities is a first cavity housing the one or more reagent, the first cavity in fluid communication with the rupturing portion,
• wherein another one of the two or more cavities is a second cavity housing a diluent which, when said inner membrane is piereced, penetrated and/or broken at approximately the same time or at a time shortly thereafter, as breaking the rupturing portion of the pouch, hydrates the one or more reagent in the first cavity having the dry formulation,
• wherein at least one of the channels has a first channel segment and a second channel segment separated by at least one of the one or more pouch assemblies such that rupturing at least two rupturing portions of the at least one pouch assembly connects the first channel segment to the second channel segment.