Composition and method for detection of demineralisation
AssigneeCalcivis Limited
InventorsEmma Perfect, Chris Longbottom
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Abstract
The invention relates to a composition for the detection of tooth demineralization. More specifically, the invention concerns a composition comprising a complex capable of producing an optical signal characteristic of the presence of free ions, the pharmaceutical uses of such a composition, and methods and a kit for the detection of active demineralization at tooth surfaces using such a composition.
Claims35
An intra-oral pharmaceutical tooth demineralization detection composition, comprising:
- a) 1 ng/mL to 10 mg/mL of a pharmaceutically acceptable concentration of proteins and protein complexes which produce a bioluminescent optical signal in the presence of free ions and
- b) a pharmaceutically acceptable concentration of an ion chelator that gives a significantly high signal to noise ratio,
- wherein the tooth demineralization detection composition is applied to at least one tooth in the mouth of a living patient.
An intra-oral pharmaceutical tooth demineralization detection composition, comprising proteins and protein complexes which produce a bioluminescent optical signal in the presence of free ions, wherein the bioluminescent optical signal is detected intra-orally, wherein the composition comprises about 1 ng to about 10 mg/mL of protein or protein complex, and wherein the protein or protein complex is modified to enhance the signal or alter its emission spectra through alteration of the DNA sequence of the gene coding for the protein or protein complex wherein the tooth demineralization detection composition is applied to at least one tooth in the mouth of a living patient.
An intra-oral pharmaceutical tooth demineralization detection composition, comprising a complex capable of producing an optical signal characteristic of the presence of free ions, wherein the complex comprises:
- a) a pharmaceutically acceptable concentration of proteins and protein complexes which produce a bioluminescent optical signal in the presence of free ions wherein the protein or protein complex comprises aequorin, obelin, clytin, mitrocomin, halistaurin, phialidin, mnemiopsin, symplectin, gr-bolinopsin, casein, calsequestrin, calexcitin, calcium binding cysteine protease, calmodulin, berovin, or mixtures thereof;
- and
- b) up to 1 mM EDTA,
- wherein the composition comprises from 1 ng/ml to 10 mg/ml of complex and gives a significantly high signal to noise ratio.
Dependencies are inferred from the claim text.