A prebiotic wipe comprising a wiping substrate and a prebiotic composition disposed thereon, the prebiotic composition comprising from about 0.5 to about 2.0 percent, by weight of the prebiotic composition, a prebiotic agent selected from the group consisting of inulin, fructo-oligosaccharide (FOS), lactulose, galacto-oligosaccharide (GOS), raffinose, stachyose, isomalto-oligosaccharide, and xylo-oligosaccharide, from about 0.01 to about 1.0 percent, by weight of the prebiotic composition, α-hydroxy acid and water, wherein the weight ratio of the α-hydroxy acid to the prebiotic agent is from about 1:100 to about 1:200 and the wipe comprises from about 500 to about 1,500 mg of the prebiotic agent and from about 10 to about 50 mg of the α-hydroxy acid per square meter of wiping substrate.