Methods of making quinoa leachates and uses thereof

A method of producing a leachate comprising 20-hydroxyecdysone from an intact quinoa seed comprising:

• contacting the intact quinoa seed with a leaching fluid to produce a leaching mixture comprising the 20-hydroxyecdysone, wherein the leaching fluid is selected from the group consisting of water, n-butanol, isopropanol, n-propanol, aqueous ethanol having a concentration less than 95% ethanol, methanol, ethyl acetate, nitromethane and formic acid, and wherein the contacting step is performed at a temperature of less than 80° C.;
• and
• separating the quinoa seed from the leaching mixture, thereby producing a leachate comprising 20-hydroxyecdysone from the quinoa seed, wherein the leachate comprises from about 100 μg to about 400 μg 20-hydroxyecdysone per gram of seed.