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US 10,441,546 B2Granted
Bacterially derived intact minicells that encompass plasmid free DNA and methods of using the same
AssigneeEnGenelC Molecular Delivery Pty Ltd
InventorsHimanshu Brahmbhatt, Jennifer MacDiarmid, Toby Hulf
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Abstract
Intact, bacterially-derived minicells can safely introduce therapeutically effective amounts of plasmid-free functional nucleic acid to target mammalian cells. To this end, functional nucleic acid can be packaged into intact minicells directly, without resort to expression constructs, the expression machinery of the host cell, harsh chemicals or electroporation.
Claims42
A composition comprising:
- (a) a plurality of intact, bacterial minicells, each minicell of the plurality comprising at least one exogenous functional nucleic acid, wherein the exogenous functional nucleic acid is DNA;
- and
- (b) a pharmaceutically acceptable carrier therefor.
A method of delivering an exogenous nucleic acid to a target cell, comprising:
- (a) providing a plurality of intact, bacterial minicells in a pharmaceutically acceptable carrier, each minicell of the plurality comprising an exogenous functional nucleic acid, wherein the exogenous functional nucleic acid is DNA;
- and
- (b) bringing the minicells into contact with a mammalian target cell such that the mammalian target cell engulfs the minicells, whereby the exogenous functional nucleic acid is released into the cytoplasm of the mammalian target cell.
Dependencies are inferred from the claim text.