Protein stabilizing factors
AssigneeBloodworks
InventorsDominic Chung, Jose Aron Lopez, Minhua Ling, Junmei Chen, Xiaoyun Fu
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Abstract
Compositions and methods are described for preventing or reducing protein loss due to protein aggregation, denaturation, and adsorption to surfaces. Also described are compositions and methods for preventing or reducing the fouling or clogging of medical devices that come into contact with blood, such as catheters. Also described are methods to treat diseases caused by activation of the microvasculature.
Claims25
A method of reducing or preventing loss of an in vitro protein by adsorption to an in vitro surface or by self-association, wherein the in vitro protein is selected from the group consisting of Von Willebrand Factor (VWF), Factor VIII, ADAMTS13, and Factor IX, the method comprising:
- a. exposing the in vitro protein to an amount of Apolipoprotein A1 (ApoA-1), high density lipoprotein (HDL), or ApoA-1 and HDL;
- and
- b. maintaining the in vitro protein in the presence of the amount of ApoA-1, HDL, or ApoA-1 and HDL and thereby reducing or preventing loss of the in vitro protein by adsorption to the in vitro surface or by self-association.
A method of reducing or preventing loss of in vitro Von Willebrand Factor (VWF) by adsorption to an in vitro surface or by self-association, the method comprising:
- a. exposing the in vitro VWF to an amount of Apolipoprotein A1 (ApoA-1), high density lipoprotein (HDL), or ApoA-1 and HDL;
- and
- b. maintaining the in vitro VWF in the presence of the amount of ApoA-1, HDL, or ApoA-1 and HDL and thereby reducing or preventing loss of the in vitro VWF by adsorption to the in vitro surface or by self-association.
A method of reducing or preventing loss of in vitro Factor VIII by adsorption to an in vitro surface or by self-association, the method comprising:
- a. exposing the in vitro Factor VIII to an amount of Apolipoprotein A1 (ApoA-1), high density lipoprotein (HDL), or ApoA-1 and HDL;
- and
- b. maintaining the in vitro Factor VIII in the presence of the amount of ApoA-1, HDL, or ApoA-1 and HDL and thereby reducing or preventing loss of the in vitro Factor VIII by adsorption to the in vitro surface or by self-association.
A method of reducing or preventing loss of in vitro ADAMTS13 by adsorption to an in vitro surface or by self-association, the method comprising:
- a. exposing the in vitro ADAMTS13 to an amount of Apolipoprotein A1 (ApoA-1), high density lipoprotein (HDL), or ApoA-1 and HDL;
- and
- b. maintaining the in vitro ADAMTS13 in the presence of the amount of ApoA-1, HDL, or ApoA-1 and HDL and thereby reducing or preventing loss of the in vitro ADAMTS13 by adsorption to the in vitro surface or by self-association.
A method of reducing or preventing loss of in vitro Factor IX by adsorption to an in vitro surface or by self-association, the method comprising:
- a. exposing the in vitro Factor IX to an amount of Apolipoprotein A1 (ApoA-1), high density lipoprotein (HDL), or ApoA-1 and HDL;
- and
- b. maintaining the in vitro Factor IX in the presence of the amount of ApoA-1, HDL, or ApoA-1 and HDL and thereby reducing or preventing loss of the in vitro Factor IX by adsorption to the in vitro surface or by self-association.
Dependencies are inferred from the claim text.