Retargeted herpesvirus with a glycoprotein H fusion
AssigneeAlma Mater Studiorum - Università di Bologna
InventorsMaria Gabriella Campadelli, Valentina Gatta
Life of this patent
Estimating the term…
Abstract
The present invention relates to the field of disease therapy. More specifically, it relates to a retargeted herpesvirus having a heterologous polypeptide fused to glycoprotein H, wherein the polypeptide targets diseased cells. It also relates to a nucleic acid comprising the genome of the herpesvirus of the invention, a vector comprising this nucleic acid and a cell comprising the nucleic acid or the vector. It further relates to killing cells using the herpesvirus of the invention and to methods for growing it in vitro.
Claims30
A recombinant infectious herpesvirus comprising a heterologous polypeptide ligand inserted into mature glycoprotein H (gH)
- (i) between amino acid 23 and amino acid 24 of the gH according to SEQ ID NO:
- 1 or a corresponding region of a homologous gH, or
- (ii) within the region starting at amino acid 116 and ending at amino acid 136 of the gH according to SEQ ID NO:
- 1 or a corresponding region of a homologous gH.
A recombinant infectious herpesvirus comprising a heterologous polypeptide ligand fused to the N-terminus of mature glycoprotein H (gH) or of a truncated gH, or inserted into gH, and further comprising a heterologous polypeptide ligand fused to the N-terminus of mature gD or of a truncated gD, or inserted into gD.
A recombinant infectious herpesvirus comprising a heterologous polypeptide ligand fused to the N-terminus of mature glycoprotein H (gH) or of a truncated gH, or inserted into gH, and further comprising an altered glycoprotein D (gD), wherein gD has an amino acid deletion starting at any of amino acid residues 26 to 33 and ending at any of amino acid residues 31 to 63, and/or starting at any of amino acid residues 65 to 86 and ending at any of amino acid residues 235 to 243 of the gD according to SEQ ID NO:
- 4 or a corresponding region of a homologous gD.
Dependencies are inferred from the claim text.