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US 10,416,052 B2Granted

In situ heat induced antigen recovery and staining apparatus and method

OwnerLee H. Angrosinventor

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Abstract

An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological sample on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to be physically removed from one apparatus to another. Each treatment step occurs within the same reaction compartment. The reaction conditions of each reaction compartment for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide.

Claims28

Dependent claims

Claim 1A method of treating biological samples · 330 words4 dependent Claim 6A method of treating biological samples · 173 words4 dependent Claim 11A method of treating biological samples · 246 words3 dependent Claim 15A method of treating biological samples · 296 words4 dependent Claim 20A method of treating biological samples · 153 words4 dependent Claim 25A method of treating biological samples · 210 words3 dependent

A method of treating biological samples, comprising:

placing a first microscope slide on a first slide support element with the first microscope slide having a first biological sample disposed thereon;
placing a second microscope slide on a second slide support element with the second microscope slide having a second biological sample disposed thereon;
horizontally moving the first slide support into a treatment chamber of an antigen recovery and staining apparatus so as to position the first microscope slide and the first biological sample in the treatment chamber;
horizontally moving the second slide support into the treatment chamber of the antigen recovery and staining apparatus so as to position the second microscope slide and the second biological sample in the treatment chamber;
treating the first biological sample by applying a first reagent to the first biological sample, heating the first reagent to a desired temperature for a desired period of time, and applying additional reagents of a treatment protocol;
treating the second biological sample by applying a second reagent to the second biological sample, heating the second reagent to a desired temperature for a desired period of time, and applying additional reagents of another treatment protocol;
determining if one of the first biological sample and the second biological sample has completed the treatment protocol;
and
removing the microscope slide with the biological sample that has completed the treatment protocol from the treatment chamber while the other biological sample continues to be treated in the treatment chamber,
wherein the step of removing the microscope slide with the biological sample that has completed the treatment protocol from the treatment chamber comprises the steps of:
horizontally moving the slide support out of the treatment chamber of the antigen recovery and staining apparatus so as to position the microscope slide with the biological sample that has completed the treatment protocol outside of the treatment chamber;
and
removing the microscope slide with the biological sample that has completed the treatment protocol from the slide support element.

10 more elements

A method of treating biological samples, comprising:

placing a first microscope slide on a first slide support element with the first microscope slide having a first biological sample disposed thereon;
horizontally moving the first slide support into a treatment chamber of an antigen recovery and staining apparatus so as to position the first microscope slide and the first biological sample in the treatment chamber;
treating the first biological sample by applying a first reagent to the first biological sample, heating the first reagent to a desired temperature for a desired period of time, and applying additional reagents of a treatment protocol;
placing a second microscope slide position on a second slide support element with the second microscope slide having a second biological sample disposed thereon;
and
horizontally moving the second slide support into the treatment chamber of the antigen recovery and staining apparatus so as to position the second microscope slide and the second biological sample in the treatment chamber while the first biological sample continues to be treated in the treatment chamber.

3 more elements

A method of treating biological samples, comprising:

placing a first microscope slide on a first slide support element with the first microscope slide having a first biological sample disposed thereon;
placing a second microscope slide on a second slide support element with the second microscope slide having a second biological sample disposed thereon;
horizontally moving the first slide support into a treatment chamber of an antigen recovery and staining apparatus so as to position the first microscope slide and the first biological sample in the treatment chamber;
horizontally moving the second slide support into the treatment chamber of the antigen recovery and staining apparatus so as to position the second microscope slide and the second biological sample in the treatment chamber;
treating the first biological sample by applying a first reagent to the first biological sample, heating the first reagent to a desired temperature for a desired period of time, and applying additional reagents of a treatment protocol;
treating the second biological sample by applying a second reagent to the second biological sample, heating the second reagent to a desired temperature for a desired period of time, and applying additional reagents of another treatment protocol;
determining if one of the first biological sample and the second biological sample has completed the treatment protocol;
and
horizontally removing the microscope slide with the biological sample that has completed the treatment protocol from the treatment chamber while the other biological sample continues to be treated in the treatment chamber.

6 more elements

A method of treating biological samples, comprising:

placing a first microscope slide on a first slide support element with the first microscope slide having a first biological sample disposed thereon;
placing a second microscope slide on a second slide support element with the second microscope slide having a second biological sample disposed thereon;
horizontally moving the first slide support into an antigen recovery and staining apparatus;
horizontally moving the second slide support into the antigen recovery and staining apparatus;
treating the first biological sample by applying a first reagent to the first biological sample, heating the first reagent to a desired temperature for a desired period of time, and applying additional reagents of a treatment protocol;
treating the second biological sample by applying a second reagent to the second biological sample, heating the second reagent to a desired temperature for a desired period of time, and applying additional reagents of another treatment protocol;
determining if one of the first biological sample and the second biological sample has completed the treatment protocol;
and
removing the microscope slide with the biological sample that has completed the treatment protocol from the antigen recovery and staining apparatus while the other biological sample continues to be treated in the antigen recovery and staining apparatus,
wherein the step of removing the microscope slide with the biological sample that has completed the treatment protocol from the antigen recovery and staining apparatus comprises the steps of:
horizontally moving the slide support out of the antigen recovery and staining apparatus so as to position the microscope slide with the biological sample that has completed the treatment protocol outside of the antigen recovery and staining apparatus;
and
removing the microscope slide with the biological sample that has completed the treatment protocol from the slide support element.

10 more elements

A method of treating biological samples, comprising:

placing a first microscope slide on a first slide support element with the first microscope slide having a first biological sample disposed thereon;
horizontally moving the first slide support into an antigen recovery and staining apparatus;
treating the first biological sample by applying a first reagent to the first biological sample, heating the first reagent to a desired temperature for a desired period of time, and applying additional reagents of a treatment protocol;
placing a second microscope slide on a second slide support element with the second microscope slide having a second biological sample disposed thereon;
and
horizontally moving the second slide support into the antigen recovery and staining apparatus so as to position the second microscope slide and the second biological sample in the antigen recovery and staining apparatus while the first biological sample continues to be treated in the antigen recovery and staining apparatus.

3 more elements

A method of treating biological samples, comprising:

placing a first microscope slide on a first slide support element with the first microscope slide having a first biological sample disposed thereon;
placing a second microscope slide on a second slide support element with the second microscope slide having a second biological sample disposed thereon;
horizontally moving the first slide support into an antigen recovery and staining apparatus;
horizontally moving the second slide support into the antigen recovery and staining apparatus;
treating the first biological sample by applying a first regent to the first biological sample, heating the first reagent to a desired temperature for a desired period of time, and applying additional reagents of a treatment protocol;
treating the second biological sample by applying a second reagent to the second biological sample, heating the second reagent to a desired temperature for a desired period of time, and applying additional reagents of another treatment protocol;
determining if one of the first biological sample and the second biological sample has completed the treatment protocol;
and
horizontally removing the microscope slide with the biological sample that has completed the treatment protocol from the antigen recovery and staining apparatus while the other biological sample continues to be treated in the antigen recovery and staining apparatus.

6 more elements

Dependencies are inferred from the claim text.

Facts

Application: 15/289,675Filed 10 Oct 2016 · continuation
Priority: 8 Jul 1999
Published: US 2017/0023449 A126 Jan 2017
Granted: 17 Sep 2019
Term ends: Not estimatedThe source records don’t support an estimate.

Classification24 codes

G01N 1/30
Preparing specimens for investigation including physical details of chemical methods covered elsewhere, e.g. G01N33/50, C12Q: staining;…
G01N 1/312
Apparatus therefor: for samples mounted on planar substrates
G01N 1/44
Preparing specimens for investigation including physical details of chemical methods covered elsewhere, e.g. G01N33/50, C12Q: sample treatment…
G01N 33/52
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing: use of…

Family

Application60/142,789Us provisional application · filed 8 Jul 1999
Application09/612,605Parent of this continuation · filed 7 Jul 2000
Application10/388,710Parent of this continuation · filed 14 Mar 2003
US 6,534,008 B1Parent of this continuation · filed 18 Mar 2003
Application10/943,394Parent of this continuation · filed 17 Sep 2004
US 6,855,292 B2Parent of this continuation · filed 15 Feb 2005
Application12/624,120Parent of this continuation · filed 23 Nov 2009
US 7,632,461 B2Parent of this continuation · filed 15 Dec 2009
Application13/311,066Parent of this continuation · filed 5 Dec 2011
US 8,071,023 B2Parent of this continuation · filed 6 Dec 2011
Application13/742,174Parent of this continuation · filed 15 Jan 2013
US 8,354,058 B2Parent of this continuation · filed 15 Jan 2013
Application14/253,555Parent of this continuation · filed 15 Apr 2014
US 8,696,988 B2Parent of this continuation · filed 15 Apr 2014
US 2017/0023449 A1Pre-grant publication · published 26 Jan 2017
US 9,464,974 B2Parent of this continuation
US 2003/0170144 A1Published application
US 2005/0054080 A1Published application
US 2010/0068096 A1Published application
US 2012/0107198 A1Published application
US 2013/0130366 A1Published application
US 2014/0227714 A1Published application
US 10,416,052 B2This patent

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