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AssigneeFarmacule Bioindustries Pty Ltd
InventorsJames Langham Dale, Robert Maxwell Harding, Douglas Kenneth Becker, Gregory John Hafner, Ilin Yang
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This invention discloses a constitutive promoter from the Taro bacilliform virus (TaBV) for expression of foreign or endogenous coding sequences in plants, including dicotyledonous and monocotyledonous plants. The invention also discloses a chimeric nucleic acid construct comprising the promoter of the invention operably linked to a foreign or endogenous polynucleotide that codes for a protein of interest or a transcript capable of modulating expression of a target gene. The invention further discloses transformed plant cells, as well as differentiated plants and plant parts, containing the construct. Methods for diagnosis and treatment of viral infections, especially badnaviral infections, are also disclosed.
A chimeric DNA construct comprising a DNA molecule having promoter activity and operably linked to a foreign or endogenous DNA sequence to be transcribed, wherein the DNA molecule has at least 95% sequence identity, or hybridizes under high stringency conditions, to a biologically active fragment of the sequence set forth in SEQ ID NO:6, wherein the biologically active fragment comprises nucleotides 527 to 970 of SEQ ID NO:6, wherein the high stringency conditions comprise 1% BSA, 1 mM EDTA, 0.5 M NaHPO4 (pH 7.2), and 7% SDS for hybridization at 65° C. and washing at 68° C. in 0.2×SSC, 0.1% SDS.
A progeny obtained from a differentiated transgenic plant comprising a chimeric construct, wherein the progeny comprises the chimeric construct, and wherein the differentiated plant is produced by a method comprising:
A plant part obtained from a differentiated transgenic plant produced by a method comprising the steps of:
A differentiated transgenic plant regenerated from transformed plant cells obtained by a method comprising the steps of:
A transformed plant cell containing a chimeric DNA construct comprising a DNA molecule having promoter activity and operably linked to a foreign or endogenous DNA sequence to be transcribed, wherein the DNA molecule has at least 95% sequence identity, or hybridizes under high stringency conditions, to a biologically active fragment of the sequence set forth in SEQ ID NO:6, wherein the biologically active fragment comprises nucleotides 527 to 970 of SEQ ID NO:6, wherein the high stringency conditions comprise 1% BSA, 1 mM EDTA, 0.5 M NaHPO4 (pH 7.2), and 7% SDS for hybridization at 65° C. and washing at 68° C. in 0.2×SSC, 0.1% SDS.
A differentiated transgenic plant comprising plant cells containing a chimeric DNA construct comprising a DNA molecule having promoter activity and operably linked to a foreign or endogenous DNA sequence to be transcribed, wherein the DNA molecule has at least 95% sequence identity, or hybridizes under high stringency conditions, to a biologically active fragment of the sequence set forth in SEQ ID NO:6, wherein the biologically active fragment comprises nucleotides 527 to 970 of SEQ ID NO:6, wherein the high stringency conditions comprise 1% BSA, 1 mM EDTA, 0.5 M NaHPO4 (pH 7.2), and 7% SDS for hybridization at 65° C. and washing at 68° C. in 0.2×SSC, 0.1% SDS.
Dependencies are inferred from the claim text.