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US 10,407,667 B2Granted
Method for rapid generation of an infectious RNA virus
AssigneesUniversite D'Aix-Marseille, Inserm (Institut National De La Sante Et De La Recherche Medicale)
InventorsFabien Aubry, Antoine Nougairéde, Gilles Quérat, Xavier De Lamballerie, Ernest Andrew Gould, Lauriane De Fabritus
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Abstract
The present invention relates to a method for rapid generation of an infectious RNA virus that completely eliminates the need of constructing a full-length c DNA, which covers the entire viral genome, cloning and propagating such full length c DNA.
Claims17
A method for generating an infectious RNA virus comprising:
- a) introducing a promoter of DNA-dependent RNA polymerase in position 5′ and optionally a terminator and a RNA polyadenylation sequence in position 3′ of the entire genome of a RNA virus;
- b) amplifying the entire viral genome as prepared in step a) including said promoter and optionally said terminator and RNA polyadenylation sequence, in at least 2, 3, 4, 5 or 6 overlapping cDNA fragments;
- c) transfecting said cDNA fragments into a host cell,
- d) incubating the host cell of step c);
- and
- e) recovering the infectious RNA virus from said incubated host cell.
3 more elements
Dependencies are inferred from the claim text.