Antibodies that inhibit long interspersed element-1 retrotransposon endonuclease activity
OwnerVictoria Perepelitsa Belancio, Mark Sokolowskiinventors
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Abstract
The present disclosure relates to monoclonal anti-LINE-1 ORF2 protein endonuclease domain antibody, and fragments and derivatives that bind the LINE-1 ORF2 protein endonuclease domain and inhibit its activity.
Claims18
A binding reagent that specifically binds to human long interspersed element-1 (L1) open reading frame 2 (ORF2) protein having an endonuclease (EN) domain without an inactivating mutation at position 205, which binding reagent is selected from the group consisting of (a) an antibody having six complementarity determining regions (CDRs), which CDRs are the same as the six CDRs of monoclonal anti-L1 ORF2 protein EN antibody (hORF2 239), (b) a fragment of said antibody, said fragment having six CDRs which are the same as the six CDRs of monoclonal anti-L1 ORF2 protein EN antibody (hORF2 239) and which retains L1 ORF2 protein EN binding activity, and (c) a derivative of said antibody, said derivative having six CDRs which are the same as the six CDRs of monoclonal anti-L1 ORF2 protein EN antibody (hORF2 239), and which retains L1 ORF2 protein EN binding activity.
A hybridoma producing a monoclonal antibody which is monoclonal anti-L1 ORF2 protein EN antibody (hORF2 239).
A method of detecting whether human long interspersed element-1 (L1) open reading frame 2 (ORF2) protein having an endonuclease (EN) domain without an inactivating mutation at position 205 is present in a sample, said method comprising of contacting said sample with a binding regent which specifically binds to human L1ORF2 protein with said EN domain, which binding reagent is selected from the group consisting of (a) an antibody having six complementarity determining regions (CDRs), which CDRs are the same as the six CDRs of monoclonal anti-L1 ORF2 protein EN domain antibody (hORF2 239), (b) a fragment of said antibody having six CDRs, which CDRs are the same as the six CDRs of monoclonal anti-L1 ORF2 protein EN domain antibody (hORF2 239), and which retains L1 ORF2 protein EN domain binding activity, and (c) a derivative of said antibody having six CDRs, which CDRs are the same as the six CDRs of monoclonal anti-L1 ORF2 protein EN domain antibody (hORF2 239), and which retains L1 ORF2 protein EN domain binding activity, under conditions which permit binding of the binding reagent to said protein in said sample, and detecting whether any binding reagent has bound to said protein in said sample.
Dependencies are inferred from the claim text.