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US 10,371,661 B2Granted
Luminescent oxygen channeling immunoassays utilizing electrochemical discharge of singlet oxygen and methods of production and use thereof
AssigneeSiemens Healthcare Diagnostics Inc.
InventorDavid J. Ledden
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Abstract
Chemiluminescent detection systems, kits, and microfluidics devices containing same, as well as methods of production and use thereof, are disclosed.
Claims17
A kit containing a chemiluminescent detection system for use with an instrument system, the chemiluminescent detection system comprising:
- (a) an electrode that specifically binds (directly or indirectly) to a target analyte, wherein the electrode has a potential applied thereto and generates singlet oxygen in its excited state;
- (b) a composition comprising a singlet oxygen-activatable chemiluminescent compound capable of directly or indirectly binding to the target analyte;
- and
- (c) a composition comprising a singlet oxygen generator;
- (d) a microfluidics device in which (a), (b), and (c) are disposed;
- and
- wherein application of the potential to (a) by the instrument system and in the presence of (c) causes generation of singlet oxygen in the absence of a light source, and wherein the singlet oxygen reacts with (b), thus generating a detectable amount of chemiluminescence;
- and
- wherein the electrode is disposed in a read chamber of the microfluidics device, and wherein the read chamber can be interrogated by the instrument system for detecting the amount of chemiluminescence produced.
6 more elements
A microfluidics device for disposal within an instrument system, the microfluidics device comprising:
- (a) an inlet channel through which a sample is applied;
- (b) at least one compartment capable of being in fluidic communication with the inlet channel, the at least one compartment containing:
- (i) an electrode that specifically binds (directly or indirectly) to a target analyte, wherein the electrode has a potential applied thereto and generates singlet oxygen in its excited state;
- (ii) a composition comprising a singlet oxygen-activatable chemiluminescent compound capable of directly or indirectly binding to the target analyte;
- and
- (iii) a composition comprising a singlet oxygen generator;
- and
- wherein application of a potential to (i) by the instrument system and in the presence of (iii) causes generation of singlet oxygen in the absence of a light source, and wherein the singlet oxygen reacts with (ii), thus generating a detectable amount of chemiluminescence;
- and
- wherein the electrode is disposed in a read chamber, and wherein the read chamber can be interrogated by the instrument system for detecting the amount of chemiluminescence produced.
7 more elements
A method for detecting the presence and/or concentration of a target analyte in a sample, comprising the steps of:
- (a) combining, either simultaneously or wholly or partially sequentially:
- (1) a sample suspected of containing the target analyte;
- (2) an electrode capable of directly or indirectly binding to the target analyte and capable of generating singlet oxygen in its excited state;
- and
- (3) a composition comprising a singlet oxygen-activatable chemiluminescent compound capable of directly or indirectly binding to the target analyte;
- (b) allowing the binding of the composition comprising the chemiluminescent compound and the electrode to the target analyte, whereby a sandwich complex is formed by the binding of (2) and (3) to the target analyte such that the electrode is brought into close proximity to the chemiluminescent compound;
- (c) washing the electrode with a composition comprising a singlet oxygen generator;
- (d) applying a potential to the electrode to generate singlet oxygen, wherein generation of singlet oxygen in close proximity to the chemiluminescent compound causes the activation of the chemiluminescent compound present in the sandwich complex, thus generating a detectable signal in the absence of a light source;
- (e) determining the amount of chemiluminescence generated by the activated chemiluminescent compound;
- (f) optionally repeating steps (b)-(e);
- and
- (g) detecting the presence and/or concentration of the target analyte by analyzing the amount of chemiluminescence so produced, wherein the amount of chemiluminescence is directly proportional to the amount of target analyte in the sample.
9 more elements
Dependencies are inferred from the claim text.