Operating method of a biological detection calibration system

A method of operating a biological detection calibration system comprising:

• a biological detection device configured to detect a biological sample disposed on a titer plate;
• a light source configured to emit a light to the biological sample that is to be detected;
• an image capturing module configured to capture a fluorescence of the biological sample generated when irradiated by the light;
• and
• a controller electrically connected to the light source and configured to drive and control the light source to emit the light, and the controller electrically connected to the image capturing module and configured to detect the biological sample;
• the method comprising:
• substituting the titer plate with a carrier, the carrier includes a plurality of recesses, where the carrier is disposed at a detecting position same as where the titer plate being disposed in the biological detection device to receive the light emitted from the light source to simulate the detection of the biological sample;
• detecting whether a light calibration device exists on the biological detection device, wherein the light calibration device comprises the carrier and a calibration sample disposed on the carrier;
• disposing the calibration sample on the carrier, the calibration sample separated and independent from the light source, the calibration sample comprises a circuit board and a light detector, wherein the circuit board is electrically connected to the light detector and the controller;
• disposing the light detector in a position corresponding to at least one of the recesses of the carrier and is configured to detect the intensity of the light directly emitted from the light source to the carrier;
• positioning the light detector on an emitting path of the light, and the calibration sample is configured to transmit an intensity information of the light detected by the light detector to the controller;
• wherein the circuit board is disposed on the carrier and the light detector is disposed to face the at least one recess of the carrier to detect the intensity of the light emitted from the light source to the recess of the carrier;
• operating the controller in a calibration mode when the carrier carrying the calibration sample substitutes the titer plate to control the light source to emit the light to the calibration sample;
• determining whether the intensity of the light detected by the light detector is less than a predetermined value;
• and
• enhancing a driving voltage of the light source, such that the intensity of the light detected by the light detector reaches the predetermined value if the intensity of the light detected by the light detector is less than the predetermined value.