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US 10,370,731 B2Granted
Compositions and methods for detection of hepatitis C virus genotype 3
AssigneeRoche Molecular Systems, Inc.
InventorRochak Mehta
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Abstract
Methods for the rapid detection of the presence or absence of Hepatitis C Virus (HCV) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting HCV and kits are provided that are designed for the detection of HCV.
Claims12
A method of detecting Hepatitis C Virus (HCV) Genotype 3 in a sample, the method comprising:
- performing an amplification step comprising contacting the sample with a set of primers to produce an amplification product if a target nucleic acid of HCV Genotype 3 is present in the sample;
- performing a hybridization step comprising contacting the amplification product with one or more detectable probe;
- and
- performing a detection step comprising detecting the presence or absence of the amplification product, wherein the presence of the amplification product is indicative of the presence of HCV Genotype 3 in the sample, and wherein the absence of the amplification product is indicative of the absence of HCV Genotype 3 in the sample;
- wherein the set of primers comprises at least a first primer comprising a nucleic acid sequence consisting of SEQ ID NO:1, or a complement thereof, and at least a second primer comprising a nucleic acid sequence consisting of SEQ ID NOs:3 or 4, or a complement thereof;
- and
- wherein the one or more detectable probes comprises a nucleic acid sequence consisting of SEQ ID NO:2, or a complement thereof.
4 more elements
Dependencies are inferred from the claim text.