Detection method for mutation in 93rd amino acid of hepatitis C virus NS5A protein, and detection kit for mutation in 93rd amino acid of hepatitis C virus NS54 protein
AssigneeSaitama Medical University
InventorsSatoshi Mochida, Yoshihito Uchida, Jun-ichi Kouyama, Kayoko Naiki
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Abstract
A method for detecting a mutation in an amino acid at position 93 of a hepatitis C virus NS5A protein, the method including: synthesizing cDNA using, as a template, hepatitis C virus RNA in a sample; and performing a real-time PCR with a cycling probe method using, as a template, the cDNA; wherein a primer set used in the real-time PCR is a certain primer set; and wherein probes used in the real-time PCR include certain probes.
Claims20
A method for detecting a mutation in an amino acid at position 93 of a hepatitis C virus NS5A protein, the method comprising:
- synthesizing cDNA using, as a template, hepatitis C virus RNA in a sample;
- and
- performing a real-time PCR with a cycling probe method using, as a template, the cDNA;
- wherein a primer set used in the real-time PCR is a primer set which is designed to be able to amplify a region comprising the base sequence coding for the amino acid at position 93 of the hepatitis C virus NS5A protein, and wherein probes used in the real-time PCR comprise:
- a probe consisting of the base sequence of SEQ ID NO:
- 1 or the complement thereof;
- and
- at least one probe selected from
- a probe consisting of the base sequence of SEQ ID NO:
- 2 or the complement thereof, and
- a probe consisting of the base sequence of SEQ ID NO:
- 18 or the complement thereof:
- (SEQ ID NO:
- 1)
- aacgcat[a]ca
- (SEQ ID NO:
- 2)
- aacgcac[a]ca
- (SEQ ID NO:
- 18)
- acgcac[a]ca
- wherein each of the SEQ ID NOs:
- 1, 2, and 18, a base described in brackets denotes RNA.
A kit for detecting a mutation in an amino acid at position 93 of a hepatitis C virus NS5A protein, the kit comprising:
- a probe consisting of the base sequence of SEQ ID NO:
- 1 or the complement thereof;
- and at least one probe selected from a probe consisting of the base sequence of SEQ ID NO:
- 2 or the complement thereof, and
- a probe consisting of the base sequence of SEQ ID NO:
- 18 or the complement thereof:
- (SEQ ID NO:
- 1)
- aacgcat[a]ca
- (SEQ ID NO:
- 2)
- aacgcac[a]ca
- (SEQ ID NO:
- 18)
- acgcac[a]ca
- wherein each of the SEQ ID NOs:
- 1, 2, and 18, a base described in brackets denotes RNA and the other bases denote DNA.
Dependencies are inferred from the claim text.