Method for detecting fungi, reaction solution for PCR, and carrier for detecting fungi

A method for detecting fungi comprising:

• preparing a PCR reaction solution comprising genomic DNA from collected fungi, a primer set for amplifying target regions of β-tubulin genes, and a primer set for amplifying target regions of ITS regions;
• wherein, in the PCR reaction solution, the concentration ratio of the primer set for amplifying the target regions of the β-tubulin genes to the primer set for amplifying the target regions of the ITS regions is 1:0.5 to 1:0.25,
• amplifying DNA fragments from the collected fungi by a multiplex PCR in the PCR reaction solution that includes a label,
• hybridizing the labeled amplification products from the multiplex PCR to a DNA chip in which a plurality of probes selected from DNA of fungi to be detected are fixed;
• and
• detecting the presence of fungi by measuring the combined label intensities of the labeled amplification product of the ITS region and the labeled amplification product of the β-tubulin gene hybridized on the DNA chip.