Antibiotic susceptibility testing using probes for preribosomal RNA
AssigneesThe Regents of the University of California, The United States of America as represented by the Department of Veterans Affairs
InventorsDavid A. Haake, Colin Halford, Jane T. Babbitt, Bernard M. Churchill
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Abstract
Described are probes and methods for detecting antibiotic susceptibility of a specimen. The method comprises contacting the specimen with an oligonucleotide probe that specifically hybridizes with a target nucleic acid sequence region of ribosomal RNA. The target sequence is at the junction between a pre-ribosomal RNA tail and mature ribosomal RNA of 23S or 16S rRNA. Performing the method in the presence and absence of an antibiotic permits detection of antibiotic susceptibility.
Claims17
A device for detecting intact pre-rRNA in a bacterial sample, the device comprising a pair of oligonucleotide probes, at least one of which is immobilized on a solid support, wherein the pair of oligonucleotide probes comprises an oligonucleotide probe up to 50 nucleotides in length that includes SEQ ID NO:
- 5 and an oligonucleotide probe up to 50 nucleotides in length that includes SEQ ID NO:
- 6, and wherein the pair of oligonucleotide probes selectively hybridizes to a target sequence specific to intact pre-rRNA, wherein the target sequence comprises contiguous nucleotides of bacterial ribosomal RNA (rRNA) spanning a splice site between pre-ribosomal RNA (prRNA) tail and mature ribosomal RNA (mrRNA).
Dependencies are inferred from the claim text.