Detection of targeted sequence regions
AssigneeGenetics Research, LLC
InventorsAnthony P. Shuber, William Glover, Thomas C. Meyers
Life of this patent
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Abstract
Provided herein are methods of detecting nucleic acids. The nucleic acid of interest may be detected by using Cas endonuclease to degrade substantially all nucleic acid in a sample except for the nucleic acid of interest, leaving the nucleic acid of interest isolated and amenable to detection. In related methods, Cas endonuclease complexes are used to protect the nucleic acid of interest while unprotected nucleic acid is digested, e.g., by exonuclease, after which the isolated nucleic acid of interest is detected.
Claims13
A method of detecting a nucleic acid, the method comprising:
- protecting, in a population of nucleic acids in a sample from an organism, first and second ends of a target nucleic acid using a first Cas endonuclease and a second Cas endonuclease, wherein the target nucleic acid is endogenous to the organism;
- digesting unprotected nucleic acids with exonuclease;
- and
- detecting the protected target nucleic acid.
A method of analyzing nucleic acid, the method comprising:
- protecting, in a population of nucleic acids, first and second ends of a target nucleic acid using a first Cas endonuclease and a second Cas endonuclease;
- digesting unprotected nucleic acids with exonuclease while the target nucleic acid is protected by the first Cas endonuclease and a second Cas endonuclease;
- and
- sequencing the target nucleic acid.
Dependencies are inferred from the claim text.