Method for cell recovery
AssigneeNissan Chemical Industries, Ltd.
InventorsAyako Aihara, Taito Nishino
Life of this patent
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Abstract
The present invention provides a method of isolating cells or tissues from a culture preparation of the cells or tissues in a medium composition which enables culture of the cells or tissues in suspension, which comprises at least one step selected from the group consisting of the following (A), (B) and (C): (A) passing the culture preparation through a filter having fine pores having a pore diameter of 5-500 μm, (B) adding a chelator to the culture preparation, and (C) diluting the culture preparation with a physiological aqueous solution.
Claims20
A method of isolating cells or tissues, which method comprises
- (A1) passing a culture preparation of the cells or tissues in a medium composition, which comprises a polymer compound having an anionic functional group that enables culture of the cells or tissues in suspension, through a filter having fine pores with a diameter of 5-500 μm,
- (A2) isolating cells or tissues from the filtrate obtained in step (A1), and
- (B) adding a chelator to the culture preparation, wherein the chelator is citric acid or a salt thereof.
A method of isolating cells or tissues, which method comprises
- (A1) passing a culture preparation of the cells or tissues in a medium composition, which comprises a polymer compound having an anionic functional group that enables culture of the cells or tissues in suspension, through a filter having fine pores with a diameter of 5-500 μm,
- (A2) isolating cells or tissues from the filtrate obtained in step (A1), and
- (C) diluting the culture preparation with a physiological aqueous solution, wherein the physiological aqueous solution is substantially free of calcium ion and magnesium ion.
Dependencies are inferred from the claim text.