Process for the enzymatic regeneration of redox cofactors

A process for the enzymatic regeneration of the redox cofactors NAD+/NADH and/or NADP+/NADPH in a one-pot reaction, wherein, as a result of at least two further enzymatically catalyzed redox reactions proceeding in the same reaction batch (product-forming reactions), one of the two redox cofactors accumulates in its reduced form and, respectively, the other one in its oxidized form, wherein

• a) in the regeneration reaction which reconverts the reduced cofactor into its original oxidized form, oxygen or a compound of general formula
• wherein R1 represents a linear-chain or branched (C1-C4)-alkyl group or a (C1-C4)-carboxy alkyl group, is reduced, and
• b) in the regeneration reaction which reconverts the oxidized cofactor into its original reduced form, a (C4-C8)-cycloalcanol or a compound of general formula
• wherein R2 and R3 are independently selected from the group consisting of H, (C1-C6) alkyl, wherein alkyl is linear-chain or branched, (C1-C6) alkenyl, wherein alkenyl is linear-chain or branched and comprises one to three double bonds, aryl, C6-C12 aryl, carboxyl, (C1-C4) carboxy alkyl, cycloalkyl, or C3-C8 cycloalkyl, is oxidized,
• wherein the redox cofactors NAD+/NADH and/or NADP+/NADPH are enzymatically regenerated in the one-pot reaction, and
• wherein the substrate(s) of the product-forming reactions are initially present in the reaction batch at a concentration of ≥3% (w/v).