Acetyl co-enzyme A carboxylase herbicide resistant plants
AssigneeColorado Wheat Research Foundation, Inc.
InventorsMichael Hal Ostlie, Scott Haley, Philip Westra, Victoria Ashley Anderson
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Abstract
The present invention provides for compositions and methods for producing crop plants that are resistant to herbicides. In particular, the present invention provides for wheat plants, plant tissues and plant seeds that contain altered acetyl-CoA carboxylase (ACCase) genes and proteins that are resistant to inhibition by herbicides that normally inhibit the activity of the ACCase protein.
Claims41
A monocot cereal plant having an acetyl-CoA carboxylase protein which confers resistance to one or more acetyl-CoA carboxylase herbicides at levels of said one or more herbicides that would inhibit the growth of said plant, wherein said acetyl-CoA carboxylase protein comprises a mutation at a position corresponding to position 2004 of the black grass reference sequence, SEQ ID NOS:
- 14 or 16.
A method for producing a plant resistant to one or more acetyl-CoA carboxylase herbicides comprising:
- a) introducing to said plant a nucleic acid sequence which encodes an ACCase protein that comprises an amino acid substitution at a position corresponding to position 2004 of the black grass reference sequence SEQ ID NO:
- 14 or 16 and wherein said ACCase protein confers resistance to inhibition by one or more acetyl-CoA carboxylase herbicides at levels of said one or more herbicides that would inhibit the growth of a plant.
A plant with a modified ACCase polynucleotide sequence, said sequence encoding an ACCase polypeptide having a valine at the position corresponding to position 2004 of the black grass reference sequence SEQ ID NO:
- 14, or 16.
A recombinant polynucleotide comprising a nucleic acid sequence selected from the group consisting of:
- (a) a nucleic acid sequence set forth in SEQ ID NO:
- 5, or 6;
- (b) a nucleic acid nucleotide sequence that encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:
- 10, 11 or 12;
- (c) a nucleic acid sequence having at least 95% sequence identity over the entire length of SEQ ID NO:
- 4, 5, or 6;
- and
- (e) a nucleic acid sequence that comprises the full length complement of any one of (a) to (d);
- wherein said nucleic acid sequence encodes a polypeptide with ACCase activity and resistance to an ACCase herbicide, wherein said polypeptide has at least 95% sequence identity to SEQ ID NO:
- 10, 11 or 12 and having a valine at a position corresponding to position 2004 of the black grass reference ACCase sequence SEQ ID NO:14, or 16;
- and
- wherein said nucleic acid sequence is operably linked to a heterologous element.
Dependencies are inferred from the claim text.