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US 10,370,659 B2Granted
Compounds and methods for increasing antisense activity
AssigneeIonis Pharmaceuticals, Inc.
InventorsXue-hai Liang, Wen Shen, Stanley T. Crooke
Life of this patent
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Abstract
In certain embodiments, the present disclosure provides compounds and methods for increasing the antisense activity of an antisense compound in a cell. In certain embodiments, the present disclosure provides methods for identifying antisense compounds having high activity. In certain embodiments, the present disclosure provides methods for identifying antisense compounds that bind to enhancer or repressor proteins.
Claims12
A method for increasing the antisense activity of an antisense compound in a cell, comprising:
- contacting the cell with an inhibitor of the amount or activity of a repressor protein that binds antisense compounds comprising a plurality of phosphorothioate internucleoside linkages in the cell;
- and subsequently contacting the cell with the antisense compound, wherein the target nucleic acid of the antisense compound does not code for the repressor protein, and wherein the antisense compound comprises a modified oligonucleotide that comprises a plurality of phosphorothioate internucleoside linkages.
Dependencies are inferred from the claim text.