Target antigen discovery, phenotypic screens and use thereof for identification of target cell specific target epitopes
AssigneeX-Body, Inc.
InventorsYan Chen, Steven M. Shamah
Life of this patent
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Abstract
The invention provides methods and compositions for identifying binding polypeptides (e.g., antibodies or antigen binding fragments thereof) that specifically binds to a cell-surface antigen. The methods of the invention generally comprise contacting a variegated nucleic acid-display library of binding polypeptides with a cell-surface antigen displayed on the exterior surface of a cell; and isolating from the library at least one library member that specifically binds to the cell-surface antigen on the exterior surface of the cell.
Claims13
A method of identifying a binding polypeptide that specifically binds to a cell-surface antigen, the method comprising:
- (a) contacting a variegated nucleic acid-display library of binding polypeptides with a cell-surface antigen naturally expressed on an exterior surface of a first cell type, wherein the variegated nucleic acid-display library is a DNA-display library, wherein each member of the DNA-display library comprises a binding polypeptide linked through an intervening DNA linker to a DNA coding sequence encoding the binding polypeptide, and wherein the DNA linker comprises a restriction endonuclease site not present in the coding sequence of members of the DNA-display library;
- (b) washing unbound library members from the first cell type;
- (c) enzymatically separating bound library members from the first cell type by cleaving the restriction endonuclease site with a suitable restriction endonuclease;
- and
- (d) isolating from the library at least one library member that specifically binds to the cell-surface antigen on the exterior surface of the first cell type, thereby identifying a binding polypeptide that specifically binds to the cell surface antigen.
Dependencies are inferred from the claim text.