LILRB2 and notch-mediated expansion of hematopoietic precursor cells
AssigneesFred Hutchinson Cancer Research Center, Board of Regents, The University of Texas System
InventorsIrwin D. Bernstein, Chengcheng Zhang, Mi Deng, Zhigang Lu, Junke Zheng
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Abstract
The current disclosure describes methods of expanding precursor cells for hematopoietic transplantation in subjects. The methods culture precursor cells in media containing an immobilized high molecular weight LILRB2 agonist or an LILRB2 agonist in combination with a Notch agonist. The expanded cells can be used to treat a variety of hematopoietic disorders.
Claims19
A method of expanding hematopoietic stem cells and hematopoietic progenitor cells ex vivo, comprising culturing said cells with (i) a Notch agonist and (ii) a leukocyte immunoglobulin-like receptor B2 (LILRB2) agonist immobilized on a first solid phase, wherein the immobilized LILRB2 agonist is an antibody to the LILRB2 receptor, or an antigen-binding fragment of said antibody.
Dependencies are inferred from the claim text.