Hematopoietic progenitor cell populations having affinity for E-selectin / L-selectin
AssigneeRobert Sackstein
InventorRobert Sackstein
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Abstract
The invention feature methods and compositions for treating hematopoietic disorders, inflammatory conditions, and cancer and providing stem cell therapy in a mammal.
Claims9
A method of increasing the engraftment potential of a hematopoietic progenitor cell population, the method comprising:
- (a) providing said hematopoietic progenitor cell population;
- and (b) contacting said hematopoietic progenitor cell population with a recombinant expression vector comprising a nucleic acid encoding one or more of CD44 polypeptide, α(2,3) sialyltransferase, α(1,3)-fucosyltransferase, or combinations thereof, wherein the nucleic acid encodes a polypeptide that increases binding activity of the hematopoietic progenitor cell population for E-selectin and/or L-selectin, thereby increasing the engraftment potential of the hematopoietic progenitor cell population.
A population of hematopoietic progenitor cells having a glycosylated polypeptide, said glycosylated polypeptide comprising a CD44 polypeptide that displays sialylated, fucosylated glycans that bind E-selectin and L-selectin, the population having a recombinant expression vector comprising a nucleic acid encoding one or more of CD44 polypeptide, α(2,3) sialyltransferase, α(1,3)-fucosyltransferase, or combinations thereof, wherein the nucleic acid increases binding activity of the population for E-selectin and/or L-selectin.
A population of ex vivo modified hematopoietic progenitor cells having HCELL activity produced by contacting, ex vivo, a hematopoietic cell population comprising hematopoietic cells having little to no HCELL activity with one or more agents that increases cell-surface expression or activity of a HCELL polypeptide on the cell surface, the agent comprising one or more of α(2,3) sialyltransferase, α(1,3)-fucosyltransferase, or combinations thereof, wherein the agent increases affinity of the population for E-selectin and/or L-selectin.
Dependencies are inferred from the claim text.