Technology for sustaining pluripotency and improved growth of stem cells in culture
AssigneeThe Regents of the University of California
InventorsPrudence Talbot, Sabrina Lin, Antonio Loza
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Abstract
A method of culturing pluripotent stem cells is provided. The method includes culturing pluripotent stem cells in a pluripotent stem cell culture medium supplemented with an additive, where the additive includes a source of acetate ions, a carboxylic acid, or a physiologically acceptable salt of the carboxylic acid, or a combination of these substances, in an amount effective to maintain the pluripotent stem cells in culture in an undifferentiated pluripotent state. Also included are pluripotent stem cell culture media and methods of making such media.
Claims6
A method for improving the culturing of pluripotent stem cells in a pluripotent stem cell (PSC) culture medium, comprising supplementing the PSC culture medium with 5 mM to 10 mM sodium acetate and culturing the pluripotent stem cells in the supplemented PSC culture medium;
- wherein the additive is effective to maintain pluripotency of the pluripotent stem cells to a greater extent as compared to control pluripotent cells cultured in the PSC culture medium not supplemented with the additive.
A method of rescuing a culture of pluripotent stems cells that has begun differentiating, comprising supplementing a PSC culture medium with 10 mM sodium acetate and incubating the culture of pluripotent stems cells in the supplemented culture medium;
- wherein the additive is effective to reduce or prevent further differentiation of the culture of pluripotent stems cells as compared to control pluripotent cells cultured in the PSC culture medium not supplemented with the additive.
A method of creating induced pluripotent stems cells, comprising supplementing a PSC culture medium with 5 mM to 10 mM sodium acetate and culturing reprogramming cells in the supplemented PSC culture medium;
- wherein the additive is effective to increase production of induced pluripotent stem cells derived from the reprogramming cells as compared to control reprogramming cells cultured in the PSC culture medium not supplemented with the additive, the source of acetate ions comprising acetic acid, a physiologically acceptable salt of acetic acid, or a metabolic precursor of acetic acid, or a combination thereof.
A method of improving a PSC culture medium, comprising supplementing the culture medium with 5 mM to 10 mM sodium acetate wherein the additive is effective to maintain pluripotency of pluripotent stem cells, reduce or prevent further differentiation of a culture of pluripotent stem cells that has begun differentiating, or increase induction of pluripotent stem cells from reprogramming cells, as compared to respective control cells cultured in the PSC culture medium not supplemented with the additive.
Dependencies are inferred from the claim text.