Antibodies to paliperidone haptens and use thereof
AssigneeJanssen Pharmaceutica NV
InventorsEric Hryhorenko, Banumathi Sankaran, Thomas R. DeCory, Theresa Tubbs, Linda Colt, Maarten Vliegen, Pieter Rik Haspeslagh
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Abstract
Disclosed is an antibody which binds to paliperidone, which can be used to detect paliperidone in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of paliperidone, including multiplex detection of aripiprazole, olanzapine, quetiapine, risperidone and paliperidone in a single lateral flow assay device.
Claims8
A method of producing an antibody or a binding fragment thereof which binds to paliperidone, the method comprising:
- (i) selecting a host for antibody production;
- and
- (ii) inoculating the host with a conjugate of a compound of Formula I and an immunogenic carrier, wherein the host produces an antibody which binds to paliperidone,
- wherein
- L1 is OC(O)(CH2)n, or O(CH2)n;
- wherein n is 2 or 3;
- L2 is NHC(O),
- or absent;
- L3 is (CH2)mCO2H, or
- wherein m is 0, 1, 2, or 3;
- provided that m may only be 0 if L2 is absent, and further provided that when L2 is absent L3 is
A method of producing a hybridoma cell line capable of producing a monoclonal antibody which binds to paliperidone, the method comprising:
- (i) selecting a host for antibody production;
- (ii) inoculating the host with a conjugate of a compound of Formula I and an immunogenic carrier;
- (iii) fusing a cell line from said inoculated host with a continuously dividing cell to create a fused cell capable of producing a monoclonal antibody which binds to paliperidone;
- and
- (iv) cloning the fused cell so as to obtain a hybridoma cell line,
- wherein
- L1 is OC(O)(CH2)n, or O(CH2)n;
- wherein n is 2 or 3;
- L2 is NHC(O),
- or absent;
- L3 is (CH2)mCO2H, or
- wherein m is 0, 1, 2, 3;
- provided that m may only be 0 if L2 is absent, and further provided that when L2 is absent L3 is
Dependencies are inferred from the claim text.