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US 10,370,440 B2Granted
Reconstituted polypeptides
AssigneeResearch Corporation Technologies, Inc.
InventorShohei Koide
Life of this patent
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Abstract
The present invention provides modified fibronectin type III (Fn3) molecules, and nucleic acid molecules encoding the modified Fn3 molecules. Also provided are methods of preparing these molecules, and kits to perform the methods.
Claims27
A fibronectin type III (Fn3) monobody binding pair comprising:
- (a) a first fibronectin type III (Fn3) monobody polypeptide comprising two to six β-strand domains selected from an A, B, C, D, E, F and G β-strand domain of the 10th Fn3 domain of fibronectin
- (i) with a loop region linked between the β-strand domains, and
- (ii) wherein a polypeptide tail region may be attached to one or both terminal β-strand domains, and
- (b) a second Fn3 monobody polypeptide comprising two to six β-strand domains selected from an A, B, C, D, E, F and G β-strand domain of the 10th Fn3 domain of fibronectin
- (i) with a loop region linked between the β-strand domains, and
- (ii) wherein a polypeptide tail region may be attached to one or both terminal β-strand domains,
- wherein the first Fn3 monobody polypeptide has a binding affinity for the second Fn3 monobody polypeptide with a dissociation constant of about 10−9 moles/liter.
4 more elements
Dependencies are inferred from the claim text.