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US 10,370,408 B2Granted
Method for purification of a DNase or RNase protein from a sample
AssigneeJHL Biotech, Inc.
InventorsGregory Scott Blank, Jiun-Liang Lin
Life of this patent
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Abstract
The present disclosure provides compositions and methods for purifying a protein such as DNase using a chromatographic process. The methods include a single chromatographic step and the use of high concentration salt buffers.
Claims25
A method for purification of a DNase or RNase from a sample comprising:
- loading a sample comprising a DNase or RNase and a detergent onto a chromatography column, washing the column with a wash buffer comprising calcium chloride, wherein the wash buffer has a conductivity of 80 mS/cm or higher, and
- eluting the DNase or RNase from the column with an elution buffer comprising calcium chloride.
Dependencies are inferred from the claim text.