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US 10,369,284 B2Granted
Kit, use thereof and method for filling connective tissue of the skin
AssigneeMeidrix Biomedicals GmbH
InventorThomas Graeve
Life of this patent
Estimating the term…
Abstract
The invention concerns a kit and a syringe for use in a therapeutic method for intracutaneous filling of the collagen structure in connective tissue of the skin, and the use thereof, particularly as part of the treatment of skin defects, particularly skin diseases and signs of skin aging.
Claims18
A method for intracutaneous filling of a collagen structure in connective tissue of a patient's skin with a collagen gel, the method comprising the steps:
- a) separately supplying a collagen solution and a neutralizing buffer solution, and separately tempering the collagen solution and the neutralizing buffer solution by bringing the collagen solution and the neutralizing buffer solution each to a temperature of 20° C. to 37° C., wherein the collagen solution comprises native collagen at a concentration of from 9 mg/ml to 16 mg/ml;
- b) mixing the tempered collagen solution and the tempered neutralizing buffer solution by merging a stream of the tempered collagen solution and a stream of the tempered neutralizing buffer solution together in a mixing device coupled to a syringe to obtain a liquid gellable collagen composition having a native collagen concentration of from 6 mg/ml to 12 mg/ml and a viscosity of 2 to 9 Pa·s;
- c) intracutaneously injecting the liquid gellable collagen composition through a needle coupled to the mixing device and into an intracutaneous target site before gelling of the liquid gellable collagen composition, the needle having an outer diameter of 0.3 to 0.4 mm;
- and
- d) shaping the injected liquid gellable collagen composition at the intracutaneous target site, wherein the shaped collagen gel cures at the target site.
2 more elements
A method for intracutaneous filling of the collagen structure in connective tissue of a patient's skin with a collagen gel, the method comprising:
- a) disposing a collagen solution comprising 9 to 16 mg/mL native collagen and having a pH of 6 or lower, and a neutralizing buffer solution comprising a buffer, a salt, and having a pH of 8.3 in separate chambers of a syringe comprising a needle having an outlet channel in fluid communication with the separate chambers by way of a mixing device, and coupled syringe plungers for emptying the separate chambers;
- b) tempering the syringe such that the collagen solution and the neutralizing buffer solution are heated to a temperature of from 20° C. to 37° C. to obtain a tempered collagen solution and a tempered neutralizing buffer solution;
- c) piercing the patient's skin with the needle and positioning the outlet channel at a target site;
- d) depressing the coupled syringe plungers, wherein the depressing causes:
- mixing of the tempered collagen solution with the tempered neutralizing buffer solution in the mixing device by expelling the tempered collagen solution and the tempered neutralizing buffer solution from their respective chambers and merging the tempered collagen solution and the tempered neutralizing buffer solution into the mixing device to obtain a liquid gellable collagen composition comprising 6 to 12 mg/mL native collagen, a pH of 7.0 to 7.5, and a viscosity of 2 to 9 Pa·s, and
- injecting the liquid gellable collagen composition intracutaneously at the target site of the patient's skin through the outlet channel of the needle, wherein the needle has an outer diameter of 0.3 to 0.4 mm;
- and
- e) molding the liquid gellable collagen composition within the patient's skin into a three-dimensional structure with a desired shape,
- wherein the liquid gellable collagen composition cures in situ within the patient's skin and retains the desired shape.
6 more elements
Dependencies are inferred from the claim text.